Optimizing protein separations with cation exchange chromatography using Agilent Buffer Advisor Protein separation with the Agilent 1260 Infinity Bio‐inert Quaternary LC System

This Technical Overview shows that the Agilent Buffer Advisor software in com‐ bination with the Agilent 1260 Infinity Bio‐inert Quaternary LC System is an ideal solution for automated protein separation by ionic strength gradients. Usually, pH scouting using premixed two‐component gradients is time‐consuming and work‐ intensive. Dynamically mixed four‐component gradients calculated by the Buffer Advisor software shorten and simplify the workflow for pH scouting. In addition, excellent retention time precision and pH consistency were gained using the gradients calculated by the Buffer Advisor software. Authors Sonja Schneider Jochen Strassner Agilent Technologies, Inc. Waldbronn, Germany RN A se A P i 9 .6 Cy to ch ro m C P i 1 0 – 10 .5 Ly so zy m P i 1 1. 35